| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
30/09/2021 |
|
Data da última atualização: |
30/09/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, V. N.; GAMA, R. N. C. S.; SANTOS, C. A. F. |
|
Afiliação: |
V. N. SANTOS, Universidade Estadual de Feira de Santana; R. N. C. S. C. GAMA, Universidade Estadual de Feira de Santana; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
|
Título: |
Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Research Society and Development, v. 20, n. 2, May. 2021. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars. MenosThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), ... Mostrar Tudo |
|
Palavras-Chave: |
SSR. |
|
Thesagro: |
Melhoramento Genético Vegetal; Spondias Tuberosa; Umbu. |
|
Thesaurus Nal: |
Plant breeding; Spondias. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226520/1/Development-and-transferability-of-microsatellite-2021.pdf
|
|
Marc: |
LEADER 02483naa a2200217 a 4500
001 2134937
005 2021-09-30
008 2021 bl uuuu u00u1 u #d
100 1 $aSANTOS, V. N.
245 $aDevelopment and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae$bSapindales), a species endemic to the Brazilian semi-arid region.$h[electronic resource]
260 $c2021
520 $aThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars.
650 $aPlant breeding
650 $aSpondias
650 $aMelhoramento Genético Vegetal
650 $aSpondias Tuberosa
650 $aUmbu
653 $aSSR
700 1 $aGAMA, R. N. C. S.
700 1 $aSANTOS, C. A. F.
773 $tResearch Society and Development$gv. 20, n. 2, May. 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
13/07/1998 |
|
Data da última atualização: |
21/07/2017 |
|
Autoria: |
EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). |
|
Título: |
CNPSo: searching for soluctions commitment to mankind. |
|
Ano de publicação: |
0 |
|
Fonte/Imprenta: |
Londrina, [199-]. |
|
Idioma: |
Inglês |
|
Notas: |
1 folder. |
|
Conteúdo: |
Technological support; Preserving nature; Technological independence; Challenges; Our people; Our coordination; National and international cooperation. |
|
Palavras-Chave: |
Brasil; Parana; Soybean. |
|
Thesagro: |
Pesquisa; Soja. |
|
Thesaurus NAL: |
Brazil; research. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/105469/1/ID-1031.pdf
|
|
Marc: |
LEADER 00688nam a2200205 a 4500
001 1444323
005 2017-07-21
008 bl uuuu u0uu1 u #d
100 1 $aEMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR).
245 $aCNPSo$bsearching for soluctions commitment to mankind.$h[electronic resource]
260 $aLondrina, [199-].$c0
500 $a1 folder.
520 $aTechnological support; Preserving nature; Technological independence; Challenges; Our people; Our coordination; National and international cooperation.
650 $aBrazil
650 $aresearch
650 $aPesquisa
650 $aSoja
653 $aBrasil
653 $aParana
653 $aSoybean
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
